5-Amino-1MQ 10mg is 5-amino-1-methylquinolinium iodide, a synthetic small-molecule quinolinium derivative produced through chemical synthesis. Each vial holds 10mg of the compound in lyophilized form as an orange solid, sealed for storage stability. 5-Amino-1MQ is characterized in laboratory research as a selective, membrane-permeable inhibitor of nicotinamide N-methyltransferase (NNMT), the enzyme that methylates nicotinamide using S-adenosylmethionine (SAM). It is studied in cultured adipocyte and muscle stem cell models of NAD+ and methyl donor metabolism and is supplied for research use only.
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5-Amino-1MQ is a quinolinium compound. Its quinoline ring system carries a methyl group on the ring nitrogen, which gives the molecule a permanent positive charge, and a primary amino group at the 5-position.
The full chemical name is 5-amino-1-methylquinolinium, and the material is indexed in PubChem under CID 66522933. It is a small molecule rather than a peptide, so it has no amino acid sequence.
It belongs to a series of N-methylated quinolinium analogues developed as NNMT inhibitors through structure-activity screening and docking to the enzyme’s nicotinamide-binding site.
Laboratory work with this NNMT inhibitor centers on enzyme selectivity assays, cofactor measurement in cultured adipocytes and myoblast differentiation in C2C12 cells. BioLongevity Labs offers 5-Amino-1MQ for sale strictly as a research compound.
Store the lyophilized compound at -20°C, sealed and protected from light and moisture.
After reconstitution, store at 2–8°C and use promptly.
Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Research Use Disclaimer
5-Amino-1MQ is supplied for research use only. It is not a drug, food, cosmetic, or dietary supplement and has not been evaluated by the FDA. By purchasing, the buyer confirms the compound will be used solely for in vitro research.
Frequently Asked Questions
What Is 5-Amino-1MQ?
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule quinolinium compound characterized in laboratory research as a selective inhibitor of the enzyme nicotinamide N-methyltransferase (NNMT). BioLongevity Labs supplies it as a 10mg lyophilized solid for research use only.
Is 5-Amino-1MQ a Peptide?
No. 5-Amino-1MQ is often listed alongside research peptides, but it is a small molecule with a quinolinium ring structure and contains no amino acid residues.
Is 5-Amino-1MQ the Same as NAD+?
No. 5-Amino-1MQ is a quinolinium compound that inhibits the NNMT enzyme, while NAD+ is a cofactor. In cultured adipocyte studies, 5-Amino-1MQ was associated with higher intracellular NAD+ and SAM levels.
What Is the CAS Number and Molecular Weight of 5-Amino-1MQ?
5-Amino-1MQ as supplied is listed under CAS number 42464-96-0 and PubChem CID 66522933. Its molecular formula is C10H11IN2, with a molecular weight of 286.11 g/mol.
How Is 5-Amino-1MQ Purity Verified?
Each 5-Amino-1MQ batch is tested by an independent third-party laboratory to a ≥99% purity specification. The Certificate of Analysis for each batch is published online and can be reviewed before purchase.
Research Overview
NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide, a precursor of NAD+. In adipose tissue models, changes in NNMT activity have been linked to shifts in SAM and NAD+ levels and to histone H3 lysine 4 methylation, placing the enzyme at a junction of methyl donor and redox cofactor metabolism [1].
Structure-activity screening of N-methylated quinolinium analogues and related ring systems identified the quinolinium scaffold as the most active series, with NNMT inhibition in the low micromolar range [2]. Docking models placed these compounds in the enzyme’s nicotinamide-binding site [2].
Follow-up work characterized 5-Amino-1MQ in parallel artificial membrane and Caco-2 permeability assays, where methylquinolinium analogues with primary amine groups showed high membrane permeability [3]. In selectivity panels, 5-Amino-1MQ did not inhibit DNMT1 or PRMT3 at up to 600 µM or NAMPT at up to 100 µM, and showed little activity against COMT and SIRT1 [3].
Two cell-based research threads build on this enzyme work.
Adipocyte Cofactor Models
In differentiated 3T3-L1 adipocytes, 5-Amino-1MQ lowered intracellular 1-methylnicotinamide, the NNMT reaction product [3]. Concentrations of 1 to 60 µM were associated with an approximately 1.2 to 1.6-fold rise in NAD+ relative to control cells, along with higher intracellular SAM at the upper concentrations [3].
The same study reported a concentration-dependent reduction in lipid accumulation in differentiating pre-adipocytes, by about 50% at 30 µM and 70% at 60 µM [3].
Muscle Stem Cell Models
In C2C12 myoblasts, 5-Amino-1MQ promoted myoblast differentiation and was associated with changes in NAD+/NADH redox state [4]. In an acute muscle injury model in 24-month-old mice, the compound was associated with greater muscle stem cell proliferation and fusion and nearly twofold larger regenerating fiber cross-sectional area [4].
A later aged-mouse study compared 5-Amino-1MQ with an exercise protocol and reported additive changes in grip strength, along with distinct proteome and metabolome shifts in skeletal muscle [5].
Tissue distribution work in mice reported high systemic exposure to 5-Amino-1MQ and distribution to adipose, muscle and liver tissue [6]. Separately, 5-AMQ has been examined in the U-2 OS and Saos-2 bone-derived cell lines and the MCC13 and MCC26 cell lines, where it was associated with reduced cell viability, higher reactive oxygen species production and apoptotic pathway activation [7].
Research Area
In Vitro Application
NNMT Enzyme Inhibition
Structure-activity and docking studies at the nicotinamide-binding site
Enzyme Selectivity
Counter-screens against DNMT1, PRMT3, COMT, NAMPT and SIRT1
Membrane Permeability
Parallel artificial membrane and Caco-2 bi-directional transport assays
NAD+ and SAM Metabolism
Cofactor and 1-methylnicotinamide measurement in 3T3-L1 adipocytes
Lipid Accumulation
Lipogenesis assays in differentiating 3T3-L1 pre-adipocytes
Myoblast Differentiation
C2C12 differentiation and NAD+/NADH redox studies
Cell Viability
Viability and reactive oxygen species assays in established cell lines
References
Kraus D, Yang Q, Kong D, et al. (2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. https://doi.org/10.1038/nature13198
Neelakantan H, Wang HY, Vance V, et al. (2017). Structure-Activity Relationship for Small Molecule Inhibitors of Nicotinamide N-Methyltransferase. Journal of Medicinal Chemistry. https://doi.org/10.1021/acs.jmedchem.7b00389
Neelakantan H, Vance V, Wetzel MD, et al. (2017). Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochemical Pharmacology. https://doi.org/10.1016/j.bcp.2017.11.007
Neelakantan H, Brightwell CR, Graber TG, et al. (2019). Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochemical Pharmacology. https://doi.org/10.1016/j.bcp.2019.02.008
Dimet-Wiley AL, Latham CM, Brightwell CR, et al. (2024). Nicotinamide N-methyltransferase inhibition mimics and boosts exercise-mediated improvements in muscle function in aged mice. Scientific Reports. https://doi.org/10.1038/s41598-024-66034-9
Pompei V, Cecati M, Serritelli EN, et al. (2025). Small Molecule Inhibitors of Nicotinamide N-Methyltransferase Enzyme for the Treatment of Osteosarcoma and Merkel Cell Carcinoma: Potential for the Development of a Targeted Therapeutic Strategy. Biomolecules. https://doi.org/10.3390/biom15111553
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Scientifically reviewed by Dr. Ky H. Le, MD. Dr. Le is a board-certified family medicine physician with over 20 years of clinical experience. Dr. Le validates the scientific accuracy of all technical content and research citations.
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