Original price was: $94.97.$94.97Current price is: $94.97.$37.99
Pancragen peptide is a synthetic tetrapeptide bioregulator with the defined sequence Lys-Glu-Asp-Trp (KEDW), produced through controlled solid-phase peptide synthesis as the free acid H-Lys-Glu-Asp-Trp-OH. It is supplied as a lyophilized white powder in a 20mg vial, with no fillers added, for stable storage and straightforward reconstitution. Pancragen belongs to the Khavinson family of short tissue-designated peptides and is characterized in laboratory work on peptide-DNA interaction, histone binding, and promoter methylation. Most published research examines it in pancreatic cell culture models, where it has been studied in relation to the expression of differentiation factors in acinar and islet cells.
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Pancragen is a four-residue peptide built from lysine, glutamic acid, aspartic acid, and tryptophan. Its low molecular weight places it among the short regulatory peptides examined for their ability to enter the nucleus and interact with chromatin.
The KEDW sequence was developed at the Saint Petersburg Institute of Bioregulation and Gerontology as part of a series of tissue-designated bioregulators. Within that series, KEDW is the sequence assigned to pancreatic research models.
Researchers should note the terminal chemistry. This product is the free acid (H-Lys-Glu-Asp-Trp-OH), while several published KEDW studies used the C-terminal amide (H-Lys-Glu-Asp-Trp-NH₂). The two forms carry different formulas and molecular weights, so record the form alongside the sequence when comparing methods.
Pancragen the bioregulator peptide is unrelated to the similarly named PancraGEN diagnostic test.
Store the lyophilized compound at -20°C, protected from light and moisture.
Let the sealed vial reach room temperature before opening to limit condensation on the powder.
After reconstitution, store at 2°C to 8°C and use promptly. For longer holding, aliquot and store at -20°C to limit freeze-thaw cycles.
Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Research Use Disclaimer
Pancragen is supplied for research use only. It is not a drug, food, cosmetic, or dietary supplement and has not been evaluated by the FDA. By purchasing, the buyer confirms the compound will be used solely for in vitro research.
Frequently Asked Questions
What Is Pancragen Peptide?
Pancragen is a synthetic tetrapeptide bioregulator with the sequence Lys-Glu-Asp-Trp (KEDW) from the Khavinson family of short peptides. It is studied in pancreatic cell culture models for its interaction with DNA, histones, and differentiation-factor expression.
What Is the Molecular Weight of Pancragen?
The free acid form of Pancragen (H-Lys-Glu-Asp-Trp-OH) has the molecular formula C₂₆H₃₆N₆O₉ and a molecular weight of 576.6 g/mol. Its monoisotopic mass is 576.2544 Da, the value used for mass spectrometry confirmation.
Is Pancragen the Free Acid or the Amidated KEDW Form?
BioLongevity Labs Pancragen is the free acid, H-Lys-Glu-Asp-Trp-OH (PubChem CID 68452877). Several published studies used the amidated form, H-Lys-Glu-Asp-Trp-NH₂ (CID 68451868, 575.6 g/mol), so the terminal chemistry should be recorded when comparing methods.
How Should Lyophilized Pancragen Be Stored?
Lyophilized Pancragen should be stored at -20°C, protected from light and moisture. After reconstitution, it is held at 2°C to 8°C and used promptly, or aliquoted and frozen to limit freeze-thaw cycles.
How Is Pancragen Purity Verified?
Pancragen is tested by an independent third-party laboratory to a purity of ≥99% by HPLC. The certificates of analysis are published online, so researchers can review the testing record before ordering.
Research Overview
Published Pancragen research centers on pancreatic cell cultures and on the molecular interactions behind the observed changes in gene expression. The work comes largely from the Saint Petersburg group and its collaborators.
In pancreatic cell cultures, Pancragen was associated with higher expression of acinar differentiation factors (Pdx1, Ptf1a) and islet differentiation factors (Pdx1, Pax6, Pax4, Foxa2, Nkx2.2) in both early-passage and late-passage cultures [1]. A related study reported increased CXCL12 and Hoxa3 expression in embryonic pancreatic cell cultures, with a stronger response in late-passage cultures [2].
In aged pancreatic cell cultures, the amidated KEDW form was associated with higher expression of MMP2, MMP9, Mcl1, and the proliferation markers PCNA and Ki67, and with lower p53 expression [3].
Several studies have examined how a four-residue peptide could influence gene expression at this level.
Epigenetic and DNA-Binding Research
Promoter methylation of the PDX1, PAX6, and NGN3 genes shifted with culture aging in pancreatic cells in step with their expression levels, and the authors propose methylation changes as one route for the age-related and KEDW-associated variation in these genes [4]. Fluorescence studies reported that KEDW binds the N-terminal regions of histones H1, H2B, H3, and H4 in a site-specific way [5].
Molecular modeling places KEDW in both the minor and major grooves of DNA, with GGCAG proposed as a putative binding sequence [6]. A later docking study of 19 short peptides assigned KEDW to an ACCT binding site [7]. A review of peptide-directed differentiation groups KEDW with the short peptides studied in pancreatic and lung cell differentiation models [8].
The table below summarizes the laboratory settings where Pancragen has been characterized.
Research Area
In Vitro Application
Cell differentiation
Expression of Pdx1, Ptf1a, Pax6, Pax4, Foxa2, and Nkx2.2 in pancreatic cell cultures
Culture aging
Comparison of differentiation and proliferation markers in early-passage and late-passage cultures
Epigenetics
Promoter methylation analysis of PDX1, PAX6, and NGN3
Chromatin binding
Peptide-histone interaction assays with H1, H2B, H3, and H4
Molecular modeling
Docking of peptide-DNA complexes at defined nucleotide motifs
References
Khavinson VKh, Durnova AO, Polyakova VO, et al. (2013). Effects of pancragen on the differentiation of pancreatic cells during their ageing. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-013-1987-6
Khavinson VKh, Linkova NS, Polyakova VO, et al. (2012). Peptides tissue-specifically stimulate cell differentiation during their aging. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-012-1664-1
Khavinson VKh, Sevost’ianova NN, Durnova AO, et al. (2012). Tetrapeptide stimulates functional activity of the pancreatic cells in aging. Advances in Gerontology. https://pubmed.ncbi.nlm.nih.gov/23734516/
Ashapkin VV, Linkova NS, Khavinson VKh, Vanyushin BF. (2015). Epigenetic mechanisms of peptidergic regulation of gene expression during aging of human cells. Biochemistry (Moscow). https://doi.org/10.1134/S0006297915030062
Fedoreyeva LI, Smirnova TA, Kolomijtseva GY, Khavinson VKh, Vanyushin BF. (2013). Interaction of short peptides with FITC-labeled wheat histones and their complexes with deoxyribooligonucleotides. Biochemistry (Moscow). https://doi.org/10.1134/S0006297913020053
Tarnovskaya SI, Yakutseni PP, Khavinson VKh. (2014). Study of interactions between DNA and tetrapeptides using methods of molecular mechanics. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-014-2426-z
Khavinson VKh, Lin’kova NS, Tarnovskaya SI. (2016). Short peptides regulate gene expression. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-016-3596-7
Khavinson V, Linkova N, Diatlova A, Trofimova S. (2020). Peptide regulation of cell differentiation. Stem Cell Reviews and Reports. https://doi.org/10.1007/s12015-019-09938-8
A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity and quality through independent laboratory testing. Every compound from BioLongevity Labs is third-party tested, and its COA is published here, so researchers can review the testing record before a compound enters a protocol.
Each COA reports results from third-party laboratory analysis, including:
Ultra-high-performance liquid chromatography with mass spectrometry (UHPLC-MS) for purity, measured against a 98% or 99% specification depending on the compound
Mass identification for molecular confirmation and content quantitation
Endotoxin testing by Limulus amebocyte lysate (LAL) or recombinant Factor C (rFC) assay, depending on the laboratory
Visual and physical characterization of the finished material
How to Verify a COA Independently
You can check every certificate against the issuing laboratory’s own records, not just the copy hosted here. Verification does not depend on BioLongevity Labs.
AV Analytics certificates carry a search code. Scan the QR code on the certificate to open the laboratory’s verification page, or enter the search code at avanalytical.com/coa-search to pull the official record.
BioRegen reports of analysis carry a Report ID and a Validation Key. Scan the QR code on the certificate to open the official record, or quote both identifiers when you contact the laboratory at the address printed on the report.
SafeCert Labs issued a number of earlier certificates. Each one carries a COA number and the signature of the reporting chemist. Quote that number when you ask the laboratory to confirm it.
Older certificates from other laboratories list their own verification details on the document.
When a batch is sent to more than one laboratory, each certificate resolves at its own issuing laboratory. A researcher can then check the results against two unrelated sources.
These COAs come from independent certified laboratories, not in-house testing. That gives researchers a verifiable record of molecular integrity for each compound. All compounds are supplied for research use only.
Scientifically reviewed by Dr. Ky H. Le, MD. Dr. Le is a board-certified family medicine physician with over 20 years of clinical experience. Dr. Le validates the scientific accuracy of all technical content and research citations.
Disclaimer: For Research Purposes Only
This content is provided strictly for research purposes and does not constitute an endorsement or recommendation for the non-laboratory application or improper handling of peptides designed for research. The information, including discussions about specific peptides and their researched benefits, is presented for informational purposes only and must not be construed as health, clinical, or legal guidance, nor an encouragement for non-research use. Peptides described here are solely for use in structured scientific study by authorized individuals. We advise consulting with research experts, medical practitioners, or legal counsel prior to any decisions about obtaining or utilizing these peptides. The expectation of responsible, ethical utilization of this information for legitimate investigative and scholarly objectives is paramount. This notice is dynamic and governs all provided content on research peptides.
Pancragen (20mg)
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Original price was: $94.97.$94.97Current price is: $94.97.$37.99
