KLOW Blend Technical Overview
KLOW Blend is a co-formulated multi-peptide research article rather than a single chemical entity. Its name is an acronym assembled from the component designations, and each constituent retains its own CAS number, PubChem record, and published research literature. A blend of this kind is used in laboratory settings where more than one signaling pathway is being examined in parallel within the same experimental system.
The four components span three structural categories. GHK-Cu is a copper(II) coordination complex of the tripeptide glycyl-L-histidyl-L-lysine. BPC-157 and KPV are short linear peptides of fifteen and three residues respectively. The TB-500 component is a 43-residue acetylated polypeptide corresponding to the beta-thymosin sequence.
Because the components differ substantially in molecular weight, from 342 g/mol for KPV to roughly 4,963 g/mol for the beta-thymosin component, the specifications below are given per component rather than as a single averaged set of values. Researchers working with the blend should handle each constituent as a separate analyte for LC-MS and HPLC verification.
Compound Specifications
The blend-level summary applies to the vial as supplied. The four sub-tables below give verified identity data for each constituent as an individual chemical entity.
Blend-Level Summary
| Property |
Value |
| Blend Composition |
GHK-Cu 50 mg, BPC-157 10 mg, TB-500 10 mg, KPV 10 mg |
| Total Peptide Content Per Vial |
80 mg |
| Component Ratio (GHK-Cu : BPC-157 : TB-500 : KPV) |
5 : 1 : 1 : 1 |
| Purity |
≥99% (HPLC), per component |
| Appearance |
Lyophilized powder, blue to blue-violet tint contributed by the copper complex |
| Solubility |
Soluble in water and in bacteriostatic water for laboratory reconstitution |
| Source |
Synthetic (solid-phase peptide synthesis) |
| Storage |
-20°C or below, protect from light |
GHK-Cu Specifications
| Property |
Value |
| CAS Number |
89030-95-5 |
| PubChem CID |
139035031 |
| Molecular Formula |
C₁₄H₂₁CuN₆O₄⁻ |
| Molecular Weight |
400.90 g/mol |
| Monoisotopic Mass |
400.0920 |
| InChIKey |
LREZPQNYQZAPJC-ACMTZBLWSA-L |
| Amino Acid Sequence |
Gly-His-Lys (GHK), copper(II) complex |
| Sequence Length |
3 residues |
| Free-Peptide Reference |
GHK free tripeptide: CAS 49557-75-7, PubChem CID 73587, C₁₄H₂₄N₆O₄, 340.38 g/mol |
| Source |
Synthetic |
| Synonyms |
Copper tripeptide-1, GHK-Cu, copper peptide, Gly-His-Lys-Cu(II), prezatide copper (free peptide: prezatide, tripeptide-1) |
BPC-157 Specifications
| Property |
Value |
| CAS Number |
137525-51-0 |
| PubChem CID |
9941957 |
| Molecular Formula |
C₆₂H₉₈N₁₆O₂₂ |
| Molecular Weight |
1419.5 g/mol |
| Monoisotopic Mass |
1418.7042 |
| InChIKey |
HEEWEZGQMLZMFE-RKGINYAYSA-N |
| Amino Acid Sequence |
GEPPPGKPADDAGLV (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val) |
| Sequence Length |
15 residues |
| ChEMBL ID |
CHEMBL4297358 |
| UNII |
8ED8NXK95P |
| Source |
Synthetic |
| Synonyms |
BPC 157, PL-14736, PLD-116, PL-10, bepecin, pentadecapeptide BPC 157 |
TB-500 Specifications
| Property |
Value |
| CAS Number |
77591-33-4 |
| PubChem CID |
16132341 |
| Molecular Formula |
C₂₁₂H₃₅₀N₅₆O₇₈S |
| Molecular Weight |
4963 g/mol |
| Monoisotopic Mass |
4960.4863 |
| Exact Mass |
4962.4930 |
| InChIKey |
UGPMCIBIHRSCBV-XNBOLLIBSA-N |
| Amino Acid Sequence |
Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH |
| Sequence Length |
43 residues |
| N-Terminal Modification |
N-terminal acetylation |
| UNII |
2D5MRE3SSY |
| Source |
Synthetic |
| Synonyms |
Timbetasin, thymosin beta 4, Tβ4, TB4, Fx peptide, WHO 10716 |
KPV Specifications
| Property |
Value |
| CAS Number |
67727-97-3 |
| PubChem CID |
125672 |
| Molecular Formula |
C₁₆H₃₀N₄O₄ |
| Molecular Weight |
342.43 g/mol |
| Monoisotopic Mass |
342.2267 |
| InChIKey |
YSPZCHGIWAQVKQ-AVGNSLFASA-N |
| Amino Acid Sequence |
KPV (Lys-Pro-Val) |
| Sequence Length |
3 residues |
| ChEBI ID |
CHEBI:160254 |
| Source |
Synthetic |
| Synonyms |
Lys-Pro-Val, alpha-MSH (11-13), ACTH (11-13), L-lysyl-L-prolyl-L-valine, tripeptide KPV |
Storage and Handling
- Store the lyophilized blend at -20°C or below, protected from light.
- After reconstitution with a sterile laboratory solvent, store aliquots at -20°C or below and use promptly.
- Aliquot before freezing to avoid repeated freeze-thaw cycles across the four components.
- Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Research Use Disclaimer
KLOW Blend is supplied for research use only. It is not a drug, food, cosmetic, or dietary supplement and has not been evaluated by the FDA. By purchasing, the buyer confirms the compound will be used solely for in vitro research.
Frequently Asked Questions
What Is KLOW Blend Made Of?
KLOW Blend contains four separately synthesized peptides in one vial: 50 mg GHK-Cu, 10 mg BPC-157, 10 mg TB-500, and 10 mg KPV, for 80 mg of total peptide content. Each component is supplied at ≥99% purity by HPLC and carries its own CAS number and PubChem record.
Which Entity Does the TB-500 Component Refer To?
The TB-500 component in KLOW Blend is the 43-residue acetylated beta-thymosin polypeptide, CAS 77591-33-4, PubChem CID 16132341. Its N-terminal tetrapeptide Ac-SDKP is a separate research entity that is generated from this sequence by prolyl oligopeptidase cleavage.
What Are the Molecular Weights of the KLOW Blend Components?
The four components range from 342.43 g/mol for KPV and 400.90 g/mol for GHK-Cu up to 1419.5 g/mol for BPC-157 and roughly 4,963 g/mol for the TB-500 component. Because the range is wide, each constituent is confirmed as a separate analyte during mass-spectrometric verification.
How Should KLOW Blend Be Stored in a Laboratory Setting?
KLOW Blend is stored lyophilized at -20°C or below and protected from light. Reconstituted material is aliquoted and kept at -20°C or below so that the four components are not subjected to repeated freeze-thaw cycles.
Why Is KLOW Blend Blue in Color?
The blue to blue-violet tint comes from the GHK-Cu component, which is a copper(II) coordination complex of the tripeptide glycyl-L-histidyl-L-lysine. The other three components are colorless peptides and do not contribute to the tint.
KLOW Blend Research Areas
GHK and its copper complex have been examined in laboratory work on extracellular matrix gene regulation. Reported observations in cultured fibroblast systems include modulation of collagen, decorin, and glycosaminoglycan synthesis alongside changes in metalloproteinase and metalloproteinase-inhibitor activity [1]. Broader transcriptional profiling work using connectivity-map methods has reported that GHK is associated with altered expression across large numbers of genes, including gene sets relevant to nervous-system function [2].
BPC-157 has been characterized in angiogenic signaling research. In endothelial cell culture and chorioallantoic membrane assays, BPC-157 exposure was associated with increased VEGFR2 expression and receptor internalization, together with activation of the VEGFR2-Akt-eNOS signaling axis; blocking endocytosis suppressed the observed tube-formation response [3]. A 2025 scoping review of the preclinical literature summarizes the same VEGFR2 and nitric oxide pathway involvement along with ERK1/2 signaling, while noting that controlled study data outside animal models remain limited [4].
The TB-500 component is studied primarily as an actin-sequestering polypeptide. Beta-thymosin binds monomeric G-actin and participates in cytoskeletal reorganization, a mechanism linked in cell-culture models to changes in migration behavior [5]. A second research thread concerns the Tβ4-POP-Ac-SDKP axis, in which prolyl oligopeptidase cleaves the parent sequence to release the N-terminal tetrapeptide Ac-SDKP, which is itself examined in fibrogenesis research models [6]. In TGF-β-stimulated lung fibroblast cultures, Ac-SDKP exposure was associated with reduced alpha-smooth muscle actin and collagen expression [7].
KPV corresponds to the C-terminal tripeptide of alpha-melanocyte-stimulating hormone. Laboratory work has reported that its observed inflammatory-signaling profile differs from that of the core melanocortin sequences and does not appear to require melanocortin receptor signaling, with interleukin-1 beta pathway involvement proposed instead [8].
Taken together, the four components map onto distinct and separately documented pathway sets, which is the reason the blend is used in multi-pathway in vitro designs.
Working With a Four-Component Blend Analytically
Because the constituents differ by more than an order of magnitude in molecular weight, purity and identity confirmation are performed per component rather than on the blend as a whole. Reversed-phase HPLC resolves the four peaks, and the InChIKey and monoisotopic mass values in the tables above allow each entity to be confirmed independently by LC-MS.
| Research Area |
In Vitro Application |
| Extracellular matrix gene regulation |
Fibroblast culture models examining collagen, decorin, and metalloproteinase expression |
| Angiogenic signaling |
Endothelial tube-formation assays examining VEGFR2, Akt, and eNOS pathway activity |
| Cytoskeletal dynamics |
Cell-culture models of actin monomer sequestration and migration behavior |
| Fibrogenesis signaling |
TGF-β-stimulated fibroblast models examining alpha-smooth muscle actin and collagen markers |
| Inflammatory signaling |
Melanocortin and interleukin-1 beta pathway assays in cultured cell lines |
| Analytical method development |
Multi-analyte HPLC and LC-MS separation and identity confirmation |
References
- Pickart L, Vasquez-Soltero JM, Margolina A. (2015). GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration. BioMed Research International. https://pmc.ncbi.nlm.nih.gov/articles/PMC4508379/
- Pickart L, Vasquez-Soltero JM, Margolina A. (2017). The Effect of the Human Peptide GHK on Gene Expression Relevant to Nervous System Function and Cognitive Decline. Brain Sciences. https://pmc.ncbi.nlm.nih.gov/articles/PMC5332963/
- Hsieh MJ, Liu HT, Wang CN, et al. (2017). Therapeutic potential of pro-angiogenic BPC157 is associated with VEGFR2 activation and up-regulation. Journal of Molecular Medicine. https://link.springer.com/article/10.1007/s00109-016-1488-y
- McGuire FP, Martinez R, Lenz A, Skinner L, Cushman DM. (2025). Regeneration or Risk? A Narrative Review of BPC-157 for Musculoskeletal Healing. Current Reviews in Musculoskeletal Medicine. https://pmc.ncbi.nlm.nih.gov/articles/PMC12446177/
- Goldstein AL, Hannappel E, Sosne G, Kleinman HK. (2012). Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opinion on Biological Therapy. https://www.tandfonline.com/doi/abs/10.1517/14712598.2012.634793
- Wang W, Jia W, Zhang C. (2022). The Role of Tβ4-POP-Ac-SDKP Axis in Organ Fibrosis. International Journal of Molecular Sciences. https://pmc.ncbi.nlm.nih.gov/articles/PMC9655242/
- Conte E, Iemmolo M, Fruciano M, et al. (2015). Effects of thymosin β4 and its N-terminal fragment Ac-SDKP on TGF-β-treated human lung fibroblasts and in the mouse model of bleomycin-induced lung fibrosis. Expert Opinion on Biological Therapy. https://www.tandfonline.com/doi/abs/10.1517/14712598.2015.1026804
- Kannengiesser K, Maaser C, Heidemann J, et al. (2008). Melanocortin-derived tripeptide KPV has anti-inflammatory potential in murine models of inflammatory bowel disease. Inflammatory Bowel Diseases. https://academic.oup.com/ibdjournal/article/14/3/324/4653598
Certificate of Analysis (COA) for Every Batch
A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity, and batch quality through independent laboratory testing. Every compound from BioLongevity Labs ships with a COA tied to its specific batch, so researchers can confirm exactly what they received before it enters a protocol.
Each COA reports results from third-party laboratory analysis, including:
- Ultra-high-performance liquid chromatography with mass spectrometry (UHPLC-MS) for purity, typically confirmed at 99% or higher
- Mass identification for molecular confirmation and content quantitation
- Endotoxin quantitation by Limulus amebocyte lysate (LAL) assay where applicable
- Visual and physical characterization of the finished material
How to verify a COA independently
Every certificate can be checked against the issuing laboratory’s own records, not just the copy hosted here. Verification does not depend on BioLongevity Labs.
- MDx BioAnalytical Laboratory certificates carry a QC tracking number and a search code. Newer certificates also carry a QR code. Scan the code, or enter the search code at mdxbiolabs.com, to pull the official record.
- BioRegen reports of analysis carry a Report ID and a Validation Key. Scan the QR code on the certificate to open the official record, or reference both identifiers when contacting the laboratory at the address printed on the report.
- SafeCert Labs certificates, which appear on a number of earlier batches, carry a COA number and the signature of the reporting chemist. Reference that number when requesting confirmation from the laboratory directly.
Batches are frequently tested by both laboratories independently. When two certificates exist for the same lot, each one resolves at its own issuing laboratory, which lets a researcher confirm the same material twice through two unrelated sources.
COAs are sourced from independent certified labs rather than in-house testing alone, giving researchers a verifiable record of molecular integrity for each batch. All compounds are supplied for research use only.
Review the COAs for this batch below, or browse the full COA library.
Klow Blend (BPC-157, TB-500, KPV,GHK-Cu) (261256)
BPC-157,TB-500, KPV With GHK-Cu (251530)
BPC-157,TB-500, KPV With GHK-Cu (251530E)
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