5-Amino-1MQ Product Description
5-Amino-1MQ is a synthetic small molecule that inhibits the enzyme nicotinamide N-methyltransferase (NNMT). By blocking NNMT, it shifts intracellular NAD+ and S-adenosylmethionine (SAM) levels, two cofactors tied to energy metabolism, redox balance, and gene regulation in laboratory models.
In cultured cells and rodent models, researchers have used 5-Amino-1MQ to study lipid metabolism, adipose tissue biology, and cellular aging pathways. This methylquinolinium derivative is a research compound of interest for fundamental studies of metabolic regulation.
BioLongevity Labs supplies 5-Amino-1MQ for research use only.
Compound Information
| Property |
Value |
| Compound Name |
5-Amino-1-methylquinolinium |
| Molecular Formula |
C10H11N2 |
| Molecular Weight |
159.21 g/mol |
| PubChem CID |
950107 |
| Compound Class |
Small-molecule NNMT inhibitor |
| Synonyms |
5-amino-1-methylquinolinium, 5-AMQ, SCHEMBL6403148, CHEMBL4116828, STL196667 |
5-Amino-1MQ Peptide Structure
Source: PubChem
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Product Usage
This product is intended as a research chemical only. This designation allows use strictly for in vitro testing and laboratory experimentation. All product information on this website is for educational purposes only. This product should be handled only by licensed, qualified professionals. It is not a drug, food, or cosmetic and may not be misbranded, misused, or mislabeled.
Research Applications
| Research Area |
Model Focus |
| NNMT enzyme inhibition |
Selectivity and membrane-permeability assays in cell-based systems |
| NAD+ and SAM metabolism |
Cofactor level measurement in cultured adipocytes |
| Lipid metabolism |
Lipogenesis assays in adipocyte cultures |
| Metabolic research models |
Body composition and glucose handling in DIO rodent models |
| Cellular proliferation |
Anti-proliferative screening in cancer cell lines |
| Pharmacokinetics |
Tissue distribution and exposure profiling in rodent models |
Storage and Handling
Store lyophilized 5-Amino-1MQ at -20°C, protected from light and moisture. In freeze-dried form, the compound stays stable for extended periods under proper storage. After reconstitution, keep refrigerated and use within a short window under sterile conditions. Handle only in a controlled laboratory setting.
5-Amino-1MQ Research
5-Amino-1-methylquinolinium (5-Amino-1MQ) is a methylquinolinium derivative built around a quinolinium core with an amino group at the 5-position and a methyl group at the 1-position. It is studied as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme linked to metabolic and oncogenic pathways in research models.
The sections below summarize what published laboratory studies report about this compound and its molecular target.
Mechanism of Action
5-Amino-1MQ inhibits NNMT, the enzyme that transfers a methyl group from SAM onto nicotinamide. NNMT sits between NAD+ metabolism and one-carbon methylation metabolism, pathways that support redox reactions, DNA repair, and transcriptional regulation. [1]
In cultured mouse adipocytes, exposure to a methylquinolinium NNMT inhibitor raised intracellular NAD+ and SAM and lowered lipogenesis. [1] Selectivity testing indicated the scaffold did not inhibit related SAM-dependent methyltransferases or NAD+ salvage enzymes. [1]
NNMT as a Metabolic Research Target
Independent research groups have studied NNMT inhibition in diet-induced obese (DIO) mouse models. In one program, a separate NNMT inhibitor reduced 1-methylnicotinamide levels and improved insulin sensitivity and glucose tolerance in DIO mice, with these effects absent in NNMT-knockout animals. [2]
In DIO mice, 5-Amino-1MQ was reported to reduce body weight and white adipose mass and to lower plasma cholesterol, without a measured change in food intake. [1] The authors framed the observed metabolic shifts as a direct effect on lipid handling in the model rather than appetite suppression. [1]
Adiposity and Microbiome Studies
Paired with a low-fat diet, 5-amino-1-methylquinolinium was used to study whole-body adiposity and weight change in DIO mice, moving several measures toward age-matched lean controls within the study window. [3] The same work profiled shifts in the cecal microbiome associated with treatment. [3]
Oncology Research Applications
Beyond metabolism, 5-Amino-1MQ has been examined in cell-based oncology research. In a cervical cancer cell line (HeLa), the compound reduced cell proliferation in a concentration- and time-dependent way and lowered expression of phospho-Akt and SIRT1, two proteins tied to tumor progression, while a non-cancer reference cell line was less affected. [4]
Pharmacokinetics and Distribution
Pharmacokinetic work in mice reported systemic exposure and distribution of 5-Amino-1MQ to metabolically active tissues, including adipose, muscle, and liver, after subcutaneous compound application. [5] In the same 28-day study, DIO mice showed reduced body weight and fat mass gains alongside improved glucose tolerance and attenuated hepatic steatosis. [5] Work on tissue-specific targeting and metabolic stability continues in research settings.
References
- Neelakantan H, Vance V, Wetzel MD, et al. (2018). Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochemical Pharmacology. https://doi.org/10.1016/j.bcp.2017.11.007
- Kannt A, Rajagopal S, Kadnur SV, et al. (2018). A small molecule inhibitor of Nicotinamide N-methyltransferase for the treatment of metabolic disorders. Scientific Reports. https://doi.org/10.1038/s41598-018-22081-7
- Dimet-Wiley A, Wu Q, Wiley JT, et al. (2022). Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice. Scientific Reports. https://doi.org/10.1038/s41598-021-03670-5
- Akar S, Duran T, Azzawri AA, et al. (2021). Small molecule inhibitor of nicotinamide N-methyltransferase shows anti-proliferative activity in HeLa cells. Journal of Obstetrics and Gynaecology. https://doi.org/10.1080/01443615.2020.1854696
- Babula JJ, Bui D, Stevenson HL, et al. (2024). Nicotinamide N-methyltransferase inhibition mitigates obesity-related metabolic dysfunction. Diabetes, Obesity & Metabolism. https://doi.org/10.1111/dom.15879
Certificate of Analysis (COA) for Every Batch
A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity, and batch quality through independent laboratory testing. Every compound from BioLongevity Labs ships with a COA tied to its specific batch, so researchers can confirm exactly what they received before it enters a protocol.
Each COA reports results from third-party laboratory analysis, including:
- High-performance liquid chromatography (HPLC) for purity, typically confirmed at 99% or higher
- Liquid chromatography mass spectrometry (LC-MS) for molecular identity and mass confirmation
- Sterility and endotoxin screening where applicable
- Chemical contaminant and residual solvent checks
COAs are sourced from independent certified labs rather than in-house testing alone, giving researchers a verifiable record of molecular integrity for each batch. All compounds are supplied for research use only.
Review the COAs for this batch below, or browse the full COA library.
5-AMINO-1MQ(251435)
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