Original price was: $279.97.$279.97Current price is: $279.97.$111.99
PNC-27 is a synthetic chimeric peptide of 32 residues that joins the p53 residue 12 to 26 segment, which carries the HDM-2 binding domain, to a 17-residue membrane-penetrating leader sequence. It is produced through controlled solid-phase peptide synthesis and supplied as a lyophilized powder in a 10mg vial with no fillers added. The peptide is characterized in laboratory work on peptide interaction with HDM-2 associated with the plasma membrane. It is studied for membrane pore formation and for selectivity between transformed and non-transformed cultured cell lines.
≥99% Purity
Third-Party Tested
COA Available
U.S. Manufactured
Quantity
In stock
Third-Party Verified Quality
≥99% purity verified through independent UHPLC-MS testing. Certificates of Analysis are available before purchase.
COAs available before purchase
U.S.-manufactured research-grade
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PNC-27 belongs to the chimeric peptide class, in which a defined protein-binding motif is fused to a cell-penetrating or membrane-residency sequence. Its amino-terminal segment, PPLSQETFSDLWKLL, reproduces residues 12 to 26 of the p53 protein. Its carboxyl-terminal segment, KKWKMRRNQFWVKVQRG, supplies the membrane-penetrating leader.
The compound is prepared by solid-phase peptide synthesis and purified by reversed-phase HPLC. It carries no post-translational modification, no fluorophore, and no conjugated carrier in this format.
Research interest in PNC-27 centers on a physical chemistry question rather than a signaling one. The peptide is examined for how a p53-derived sequence adopts an HDM-2 binding conformation and what happens at a lipid bilayer when that binding partner is present in the membrane rather than the nucleus.
Soluble in water; aqueous solubility of ≥100 mg/mL has been reported for the free peptide
Storage
-20°C, protect from light
Storage and Handling
Store the lyophilized compound at -20°C in the sealed vial, protected from light and moisture.
After reconstitution, store at 2°C to 8°C and use promptly; for longer holds, aliquot and store at -20°C to avoid repeated freeze-thaw cycles.
Allow the vial to reach ambient temperature before opening so condensation does not enter the powder.
Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
PNC-27 is a 32-residue chimeric peptide that joins residues 12 to 26 of p53, the segment that binds HDM-2, to a membrane-residency sequence from penetratin. NMR work in aqueous and membrane-mimetic solvents found that it folds into amphipathic helix-coil-helix structures, with its hydrophobic residues gathered on one face [1].
The p53 residues of that structure superimpose on the same residues bound to HDM-2, which pointed researchers toward HDM-2 as the peptide’s binding partner at the cell surface [2]. HDM-2 was then detected in the membranes of several transformed cell lines but not in untransformed lines, and fluorescent labeling placed PNC-27 together with the membrane-bound protein [2].
Untransformed MCF-10-2A cells became susceptible to PNC-27 once they were made to express full-length HDM-2 with a membrane-localization signal [2]. A double-labeled form of the peptide stayed intact in MCF-7 cell membranes during lysis, while the untransformed MCF-10-2A line degraded it and remained viable [3].
The same dependence on membrane HDM-2 has been reported in the SKOV-3 and OVCAR-3 lines, with no colocalization or cytotoxicity in HUVEC controls [4]. Work in the p53-null K562 line points to a mechanism that does not rely on p53 activity inside the cell [5].
Later studies looked more closely at how the pores themselves assemble.
Pore Formation at the Plasma Membrane
Immuno-scanning electron microscopy found gold-labeled PNC-27 and HDM-2 in roughly 1:1 ratios within ring-shaped structures lining pores near the cell surface, and no pores formed in untransformed fibroblasts exposed to the peptide [6]. Pore assembly has been described as a temperature-independent 1:1 binding step followed by a temperature-dependent dimerization of those complexes [7].
In the MIA-PaCa-2 cell line, a monoclonal antibody against the p53 binding site of HDM-2 (residues 1 to 109) blocked PNC-27-induced necrosis, and immunogold labeling also placed the peptide on mitochondrial membranes [8].
Research Area
In Vitro Application
Peptide Conformation
NMR analysis of amphipathic helix-coil-helix folding in aqueous and membrane-mimetic solvents
Membrane Protein Binding
Colocalization assays of PNC-27 with plasma membrane HDM-2 in cultured cell lines
Pore Formation
Immuno-electron microscopy of transmembrane pore assembly alongside LDH release assays
Cell-Line Selectivity
Comparison of transformed lines with untransformed controls such as MCF-10-2A and HUVEC
p53-Independent Mechanisms
Membrane lysis studies in p53-null lines such as K562
Mitochondrial Membranes
Organelle dye retention and immunogold labeling of mitochondrial membranes
References
Rosal R, Pincus MR, Brandt-Rauf PW, et al. (2004). NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein… Biochemistry. https://doi.org/10.1021/bi035718g
Sarafraz-Yazdi E, Bowne WB, Adler V, et al. (2010). …peptide PNC-27 adopts an HDM-2-binding conformation… by binding to HDM-2 in their membranes. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.0909364107
Sookraj KA, Bowne WB, Adler V, et al. (2010). The… peptide, PNC-27, induces… cell lysis as the intact peptide. Cancer Chemotherapy and Pharmacology. https://doi.org/10.1007/s00280-009-1166-7
Thadi A, Gleeson EM, Khalili M, et al. (2020). …Cell Necrosis of Epithelial Ovarian… Cell Lines Depends on High Expression of HDM-2 Protein in Their Membranes. Annals of Clinical and Laboratory Science. https://pubmed.ncbi.nlm.nih.gov/33067207/
Davitt K, Babcock BD, Fenelus M, et al. (2014). The… peptide, PNC-27, induces… cell necrosis of a poorly differentiated non-solid tissue… cell line that depends on expression of HDM-2 in the plasma membrane of these cells. Annals of Clinical and Laboratory Science. https://pubmed.ncbi.nlm.nih.gov/25117093/
Sarafraz-Yazdi E, Mumin S, Cheung D, et al. (2022). PNC-27, a Chimeric p53-Penetratin Peptide Binds to HDM-2 in a p53 Peptide-like Structure, Induces Selective Membrane-Pore Formation… Biomedicines. https://doi.org/10.3390/biomedicines10050945
Pincus MR, Silberstein M, Zohar N, Sarafraz-Yazdi E, Bowne WB. (2024). Poptosis or Peptide-Induced Transmembrane Pore Formation… Biomedicines. https://doi.org/10.3390/biomedicines12061144
Krzesaj P, Adler V, Feinman RD, et al. (2024). …Peptide PNC-27… Unique Interactions with Plasma Membrane-Bound hdm-2 and with Mitochondrial Membranes Causing Mitochondrial Disruption. Annals of Clinical and Laboratory Science. https://pubmed.ncbi.nlm.nih.gov/38802154/
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Endotoxin testing by Limulus amebocyte lysate (LAL) or recombinant Factor C (rFC) assay, depending on the laboratory
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Disclaimer: For Research Purposes Only
This content is provided strictly for research purposes and does not constitute an endorsement or recommendation for the non-laboratory application or improper handling of peptides designed for research. The information, including discussions about specific peptides and their researched benefits, is presented for informational purposes only and must not be construed as health, clinical, or legal guidance, nor an encouragement for non-research use. Peptides described here are solely for use in structured scientific study by authorized individuals. We advise consulting with research experts, medical practitioners, or legal counsel prior to any decisions about obtaining or utilizing these peptides. The expectation of responsible, ethical utilization of this information for legitimate investigative and scholarly objectives is paramount. This notice is dynamic and governs all provided content on research peptides.
PNC-27 (10mg)
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Original price was: $279.97.$279.97Current price is: $279.97.$111.99
