TB-500 Product Description
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring 43 amino acid peptide found in virtually all mammalian cells. Thymosin Beta-4 acts as a multifunctional regulatory peptide comprising distinct bioactive regions within its structure: the N-terminal tetrapeptide (Ac-SDKP) mediates anti-inflammatory and antifibrotic activities, amino acids 1-15 inhibit apoptosis and promote cell survival, while the central actin-binding domain (amino acids 17-23, containing LKKTET) drives angiogenesis, cell migration, and wound healing.
Thymosin beta-4 is among the most abundant beta-thymosins found in mammalian tissue and is largely unstructured in aqueous solution. That conformational flexibility is central to how it is studied: an unfolded backbone can recognize multiple molecular targets, which is the working explanation for the wide range of binding partners reported for it.
Two sequence regions do most of the mechanistic work in the literature. The central actin-binding motif KLKKTET, spanning residues 17 through 23, mediates G-actin sequestration. The N-terminal tetrapeptide AcSDKP is a separately characterized fragment with its own experimental record.
Compound Information
| Property | Value |
|---|---|
| Synonyms | Thymosin Beta-4; Thymosin β4; Tβ4; TB4; Timbetasin (INN); TMSB4X gene product |
| CAS Number | 77591-33-4 |
| PubChem CID | 45382195 |
| Molecular Formula | C₂₁₂H₃₅₀N₅₆O₇₈S |
| Molecular Weight | 4963.44 g/mol |
| Amino Acid Sequence | Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES |
| Sequence Length | 43 residues, N-terminally acetylated |
| Actin-Binding Motif | KLKKTET (residues 17 to 23) |
| Source | Synthetic |
| InChIKey | UGPMCIBIHRSCBV-XNBOLLIBSA-N |
| Purity | ≥99% (HPLC) |
| Appearance | Lyophilized white powder |
| Solubility | Soluble in water |
| Storage | -20°C, protect from light |
TB-500 (Thymosin Beta-4) Peptide Structure
Source: PubChem
Storage and Handling
- Store the lyophilized compound at -20°C, sealed and protected from light.
- After reconstitution, store at 2°C to 8°C and use promptly.
- Allow the vial to reach room temperature before opening to limit moisture uptake.
- Brief sonication may be required for complete dissolution at higher working concentrations.
- Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
