Original price was: $79.97.$79.97Current price is: $79.97.$31.99
Thymagen is a synthetic dipeptide of the sequence L-glutamyl-L-tryptophan, assembled through controlled peptide synthesis rather than tissue extraction. Each vial contains 20mg of the compound supplied as a lyophilized white powder for storage and handling stability. Supplied at ≥99% purity by HPLC for controlled laboratory research.
≥99% Purity
Third-Party Tested
COA Available
U.S. Manufactured
Quantity
In stock
Third-Party Verified Quality
≥99% purity verified through independent UHPLC-MS testing. Certificates of Analysis are available before purchase.
COAs available before purchase
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Thymagen belongs to the short peptide bioregulator group associated with the work of V. Kh. Khavinson and colleagues, a family of two-to-four residue sequences originally separated from animal tissue homogenates and later reproduced synthetically. At two residues it is one of the shortest members of that group, with a free glutamate side chain at one end and an indole ring at the other.
Naming around this compound is inconsistent across suppliers, so the identity is worth stating plainly. Thymagen, Thymogen, Oglufanide, Timogen and the EW dipeptide are all names for the same L-α-glutamyl-L-tryptophan molecule. It is not Thymalin, which is a multi-component thymus peptide complex rather than a single defined sequence, and it is not Thymodepressin, which is built from the D-form amino acids and behaves differently in the same assay systems.
The compound is characterized in laboratory research covering peptide-DNA interaction modeling, cytokine expression in cultured cell lines, and enzyme activity assays. BioLongevity Labs supplies it strictly as a reference material for in vitro work.
Thymogen, Oglufanide, Timogen, EW dipeptide, H-Glu-Trp-OH, L-α-glutamyl-L-tryptophan, IM 862 dipeptide, NSC-334073
Purity
≥99% (HPLC)
Appearance
Lyophilized white powder
Storage
-20°C, protect from light
Storage and Handling
Store the lyophilized compound at -20°C, protected from light and moisture.
After reconstitution, store at -20°C in single-use aliquots and use promptly. Avoid repeated freeze-thaw cycles.
Allow sealed vials to equilibrate to room temperature before opening to limit condensation on the powder.
Maintain aseptic handling throughout to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Research Use Disclaimer
Thymagen 20mg is supplied for research use only. It is not a drug, food, cosmetic, or dietary supplement and has not been evaluated by the FDA. By purchasing, the buyer confirms the compound will be used solely for in vitro research.
Frequently Asked Questions
What Is Thymagen?
Thymagen 20mg is a vial containing 20 milligrams of the synthetic dipeptide L-glutamyl-L-tryptophan, supplied as a lyophilized powder for laboratory research. The same molecule is indexed under PubChem CID 100094 and CAS 38101-59-6.
Is Thymagen the Same Compound as Thymogen?
Yes. Thymagen and Thymogen are alternate spellings for the same L-α-glutamyl-L-tryptophan dipeptide, which also appears in the literature as Oglufanide and as the EW dipeptide. It is a different entity from Thymalin, a multi-component thymus peptide complex.
What Is the Molecular Weight and Sequence of Thymagen?
Thymagen has a molecular weight of 333.34 g/mol and a molecular formula of C₁₆H₁₉N₃O₅. The sequence is two residues, Glu-Trp, written EW in one-letter notation.
How Is Thymagen Purity Verified and What Documentation Ships With It?
Thymagen supplied by BioLongevity Labs is tested to ≥99% purity by HPLC, with mass confirmation against the compound’s monoisotopic mass of 333.13247 Da. A third-party Certificate of Analysis accompanies each lot.
How Should Thymagen 20mg Be Stored and Reconstituted?
Store the sealed lyophilized vial at -20°C away from light. Reconstitution solvent is chosen by the laboratory to suit the assay, noting that the free dipeptide has been reported as soluble in DMSO at 34 mg/mL with low aqueous solubility.
Research on Thymagen
Scientific literature reveals Thymagen’s complex interactions with immune cells, DNA sequences, and cellular signaling cascades in laboratory models. Research data below supports experimental design for studies investigating immunomodulation, aging, and therapeutic applications in controlled laboratory settings.
Immunomodulatory Functions
Thymagen operates through sophisticated mechanisms that regulate cellular and humoral immunity. The peptide activates TLR and RLR pathways, fundamental components of innate and adaptive immune responses[1].
This activation stimulates the expression of endosomal receptors TLR3, TLR7, TLR8, and TLR9, along with cytoplasmic sensors RIG1 and MDA5. The peptide also enhances critical signaling factors including NFκB1 and MAVS (mitochondrial antiviral signaling protein)[1].
The immunoprotective effects manifest through restoration of T and B lymphocyte populations and their subpopulations[2]. Thymagen demonstrates particular efficacy in stimulating the differentiation of hematopoietic stem cells into mature CD28+ T lymphocytes[3].
Research demonstrates that Thymagen significantly affects cytokine production patterns. In lipopolysaccharide-induced inflammation models, the peptide reduces TNF-α synthesis by approximately five-fold and moderates other pro-inflammatory mediators[3].
This cytokine regulation occurs through the peptide’s ability to interact with specific DNA sequences and influence gene expression patterns related to inflammatory responses[3].
Molecular Mechanisms
Thymagen exhibits selective binding affinity for double-stranded DNA sequences. Molecular modeling studies identify GGAG as the optimal binding site in the classical B-form of DNA[3].
This interaction occurs through the formation of intermolecular hydrogen bonds within the minor groove of the DNA helix. The peptide forms four intermolecular hydrogen bonds with nitrogen atoms at specific guanine and adenine positions.
The peptide’s DNA-binding capability translates into regulation of genes associated with immune function and inflammatory responses. Bioinformatics analysis reveals that Thymagen can potentially regulate 542 genes whose promoters contain the GGAG sequence[3].
Among these targets are critical genes involved in viral pathogenesis, including ACE2, CYSLTR1, AKT1, and AKT2. The mechanism involves epigenetic regulation where Thymagen binding influences chromatin structure and transcription factor accessibility[2].
Cardiovascular and Vascular Research
Thymagen demonstrates significant effects on the renin-angiotensin system through its interaction with ACE2. The peptide reduces angiotensin-induced vasoconstriction and preserves endothelium-dependent vascular relaxation[4].
This mechanism becomes particularly relevant in viral infections where ACE2 serves as a cellular entry point for pathogens like SARS-CoV-2[3].
Studies reveal that Thymagen influences blood coagulation and fibrinolytic activity. The peptide helps normalize plasma fibrinolytic parameters and reduces hypercoagulation states in various pathological conditions[2].
This hemostatic regulation occurs through modulation of tissue factor expression, plasminogen activators, and antithrombin III synthesis.
Cellular Biology and Aging Research
Thymagen promotes cellular differentiation processes, particularly in immune cell populations. The peptide stimulates the expression of CD4 and CD5 molecules on thymic cells, facilitating their differentiation toward T-helper phenotypes[2].
In organotypic culture studies, Thymagen increases the growth zone index of spleen tissue explants by 20-50% and stimulates proliferation while reducing apoptosis by 29-42%[2].
Research demonstrates geroprotective effects of Thymagen in both animal experiments and clinical studies. The peptide contributes to restoration of immune system functions and reduced mortality in elderly populations[2].
These anti-aging effects correlate with the peptide’s ability to regulate telomere length in PHA-stimulated lymphocytes and influence aging-related gene expression. The mechanism involves interaction with histone proteins that affect chromatin conformation and gene accessibility[2].
Respiratory and Viral Defense
Thymagen accumulates in epithelial cells of the human respiratory tract during early embryogenesis. Clinical applications include treatment of acute respiratory diseases, where the peptide reduces disease incidence by 2.0-2.4 times in elderly populations[2].
The peptide’s antiviral properties stem from its ability to activate innate immune responses through TLR/RLR pathways. This activation enhances cellular capacity to detect and respond to viral pathogens[1].
In COVID-19 research, Thymagen demonstrates efficacy in complex therapy protocols. The peptide helps normalize lymphocyte counts and monocyte populations during coronavirus infection[5].
Neurological and Stress Response
Thymagen influences stress response pathways through its effects on the hypothalamic-pituitary-adrenal axis. The peptide helps normalize stress-induced immune dysfunction and supports cellular resistance to various stressors[2].
This stress-protective effect involves regulation of heat shock protein synthesis and cellular stress response genes.
While primarily recognized for immune functions, Thymagen demonstrates indirect neuroprotective effects. The peptide’s anti-inflammatory properties help maintain neurological function during inflammatory states[2].
Tissue Regeneration
Thymagen promotes regeneration processes in various tissues through its effects on cellular proliferation and differentiation. The peptide normalizes regeneration processes when suppressed due to pathological conditions or aging[2].
This regenerative capacity involves stimulation of stem cell differentiation and enhancement of tissue repair mechanisms.
References
T. M. Sokolova, V. V. Poloskov, A. N. Shuvalov, O. S. Burova, and Z. A. Sokolova, “Signaling TLR/RLR-mechanisms of immunomodulating action of ingavirin and thymogen preparations,” Publishing House ABV Press, Apr. 2019. doi: 10.17650/1726-9784-2019-18-1-60-66. https://doi.org/10.17650/1726-9784-2019-18-1-60-66
V. Kh. Khavinson, N. S. Linkova, N. I. Chalisova, and O. M. Ivko, “The Use of Thymalin for Immunocorrection and Molecular Aspects of Biological Activity,” Pleiades Publishing Ltd, Jul. 2021. doi: 10.1134/s2079086421040046. https://doi.org/10.1134/s2079086421040046
N. Linkova et al., “The Influence of KE and EW Dipeptides in the Composition of the Thymalin Drug on Gene Expression and Protein Synthesis Involved in the Pathogenesis of COVID-19,” MDPI AG, Aug. 2023. doi: 10.3390/ijms241713377. https://doi.org/10.3390/ijms241713377
G. Deplanque et al., “Phase II trial of the antiangiogenic agent IM862 in metastatic renal cell carcinoma,” Springer Science and Business Media LLC, Oct. 2004. doi: 10.1038/sj.bjc.6602126. https://doi.org/10.1038/sj.bjc.6602126
B. Kuznik et al., “Peptide Drug Thymalin Regulates Immune Status in Severe COVID-19 Older Patients,” Pleiades Publishing Ltd, Oct. 2021. doi: 10.1134/s2079057021040068. https://doi.org/10.1134/s2079057021040068
A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity and quality through independent laboratory testing. Every compound from BioLongevity Labs is third-party tested, and its COA is published here, so researchers can review the testing record before a compound enters a protocol.
Each COA reports results from third-party laboratory analysis, including:
Ultra-high-performance liquid chromatography with mass spectrometry (UHPLC-MS) for purity, measured against a 98% or 99% specification depending on the compound
Mass identification for molecular confirmation and content quantitation
Endotoxin testing by Limulus amebocyte lysate (LAL) or recombinant Factor C (rFC) assay, depending on the laboratory
Visual and physical characterization of the finished material
How to Verify a COA Independently
You can check every certificate against the issuing laboratory’s own records, not just the copy hosted here. Verification does not depend on BioLongevity Labs.
AV Analytics certificates carry a search code. Scan the QR code on the certificate to open the laboratory’s verification page, or enter the search code at avanalytical.com/coa-search to pull the official record.
BioRegen reports of analysis carry a Report ID and a Validation Key. Scan the QR code on the certificate to open the official record, or quote both identifiers when you contact the laboratory at the address printed on the report.
SafeCert Labs issued a number of earlier certificates. Each one carries a COA number and the signature of the reporting chemist. Quote that number when you ask the laboratory to confirm it.
Older certificates from other laboratories list their own verification details on the document.
When a batch is sent to more than one laboratory, each certificate resolves at its own issuing laboratory. A researcher can then check the results against two unrelated sources.
These COAs come from independent certified laboratories, not in-house testing. That gives researchers a verifiable record of molecular integrity for each compound. All compounds are supplied for research use only.
Scientifically reviewed by Dr. Ky H. Le, MD. Dr. Le is a board-certified family medicine physician with over 20 years of clinical experience. Dr. Le validates the scientific accuracy of all technical content and research citations.
Disclaimer: For Research Purposes Only
This content is provided strictly for research purposes and does not constitute an endorsement or recommendation for the non-laboratory application or improper handling of peptides designed for research. The information, including discussions about specific peptides and their researched benefits, is presented for informational purposes only and must not be construed as health, clinical, or legal guidance, nor an encouragement for non-research use. Peptides described here are solely for use in structured scientific study by authorized individuals. We advise consulting with research experts, medical practitioners, or legal counsel prior to any decisions about obtaining or utilizing these peptides. The expectation of responsible, ethical utilization of this information for legitimate investigative and scholarly objectives is paramount. This notice is dynamic and governs all provided content on research peptides.
Thymagen (Thymogen) (20mg)
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Original price was: $79.97.$79.97Current price is: $79.97.$31.99
