Original price was: $69.97.$69.97Current price is: $69.97.$27.99
Bronchogen 20mg is a synthetic tetrapeptide bioregulator with the sequence Ala-Glu-Asp-Leu (AEDL), produced through controlled peptide synthesis. Each vial holds 20mg of Bronchogen peptide as a lyophilized white powder, freeze-dried for storage stability. Bronchogen belongs to the Khavinson series of short regulatory peptides. It is characterized in laboratory research on bronchial epithelial gene expression and DNA and peptide interaction, and is supplied for research use only.
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Bronchogen is a four-residue peptide built from alanine, glutamic acid, aspartic acid and leucine. Researchers often refer to it by its one-letter code, AEDL, and it is indexed in PubChem under CID 11690869.
It is one of the short peptide bioregulators developed at the St. Petersburg Institute of Bioregulation and Gerontology. These di-, tri- and tetrapeptides are studied for tissue-specific effects on gene expression in cell culture models.
Laboratory work on the Bronchogen peptide centers on bronchial epithelial cell cultures, DNA binding assays and promoter methylation analysis. Its small size and four charged or hydrophobic side chains make it a convenient model compound for studying how short peptides interact with nucleic acids.
Store the lyophilized compound at -20°C, sealed and protected from light and moisture.
After reconstitution, store at 2–8°C and use promptly.
Maintain aseptic handling to preserve compound integrity.
Lyophilized Format
This compound ships in lyophilized (freeze-dried) form. Freeze-drying supports long-term storage stability and preserves compound integrity. No fillers are added.
Research Use Disclaimer
Bronchogen is supplied for research use only. It is not a drug, food, cosmetic, or dietary supplement and has not been evaluated by the FDA. By purchasing, the buyer confirms the compound will be used solely for in vitro research.
Frequently Asked Questions
What Is Bronchogen Peptide?
Bronchogen is the synthetic tetrapeptide Ala-Glu-Asp-Leu (AEDL), a short peptide bioregulator from the Khavinson series studied in bronchial epithelial cell research. BioLongevity Labs supplies it as a 20mg lyophilized powder for research use only.
What Is the Amino Acid Sequence of Bronchogen?
Bronchogen has the sequence Ala-Glu-Asp-Leu, written AEDL in one-letter code. Some publications list the order as Ala-Asp-Glu-Leu (ADEL), so researchers comparing studies should check the sequence each paper reports against PubChem CID 11690869.
What Is the CAS Number and Molecular Weight of Bronchogen?
Bronchogen is listed under CAS number 857267-12-0 and PubChem CID 11690869. Its molecular formula is C18H30N4O9, with a molecular weight of 446.5 g/mol and a monoisotopic mass of 446.2013 Da.
How Should Bronchogen 20mg Be Stored?
Bronchogen 20mg should be kept sealed at -20°C in its lyophilized form, away from light and moisture. Once reconstituted, it should be held at 2–8°C and used promptly.
How Is Bronchogen Purity Verified?
Each Bronchogen batch is tested by an independent laboratory to a ≥99% purity specification. The Certificate of Analysis for each batch is published before purchase.
Research Overview
In cultured bronchial epithelial cells, Bronchogen has been associated with changes in the expression of NKX2-1, SCGB1A1, SCGB3A2, FOXA1 and FOXA2, genes linked to epithelial differentiation [1]. The same study reported shifts in Ki67, Mcl-1, p53, CD79 and NOS-3 protein levels across early and late culture passages, with the largest proliferation marker changes in late-passage cultures [1].
Follow-up work examined promoter methylation in aging bronchial cell cultures. Methylation of the NKX2-1 and SCGB1A1 promoter regions changed with culture age in step with their expression levels, which points to an epigenetic layer in the observed gene activity [2]. Earlier work reported that Bronchogen raised expression of the differentiation factors CXCL12 and Hoxa3 in bronchial cell cultures, again most clearly in aged passages [3].
A second thread of research looks at how the peptide interacts with DNA itself.
DNA Binding and Nucleic Acid Interaction
In fluorescence quenching assays, Bronchogen bound preferentially to deoxyribooligonucleotides containing CTG sequences, which sit within CNG sites that serve as cytosine methylation targets [4]. Calorimetry showed the peptide raised the melting temperature of calf thymus and mouse liver DNA by 3.1°C within a narrow peptide to base-pair ratio, without adenine-thymine or guanine-cytosine selectivity [5].
Molecular docking models place AEDL at a ctcc binding sequence [6], and spectroscopy in the bronchial epithelium study located a binding region in the major groove at guanine N7 [1].
Beyond cell culture, Bronchogen has been examined in a rodent model of airway remodeling induced by intermittent nitrogen dioxide exposure, where Bronchogen groups showed lower neutrophilic inflammatory markers and higher secretory IgA in bronchoalveolar lavage fluid [7]. Computational docking has also ranked AEDL among the stronger short-peptide ligands of the LAT1, LAT2 and PEPT1 transporters, a possible route of cellular uptake [8].
Promoter methylation analysis across early and late culture passages
DNA and Peptide Interaction
Thermal stability, spectroscopy and oligonucleotide binding assays
Cell Proliferation
Ki67 and Mcl-1 marker studies in aging cell cultures
Membrane Transport
Docking studies at LAT1, LAT2 and PEPT1 transporters
References
Khavinson VKh, Tendler SM, Vanyushin BF, et al. (2014). Peptide regulation of gene expression and protein synthesis in bronchial epithelium. Lung. https://doi.org/10.1007/s00408-014-9620-7
Ashapkin VV, Linkova NS, Khavinson VKh, Vanyushin BF. (2015). Epigenetic mechanisms of peptidergic regulation of gene expression during aging of human cells. Biochemistry (Moscow). https://doi.org/10.1134/S0006297915030062
Khavinson VKh, Linkova NS, Polyakova VO, et al. (2012). Peptides tissue-specifically stimulate cell differentiation during their aging. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-012-1664-1
Fedoreyeva LI, Kireev II, Khavinson VKh, Vanyushin BF. (2011). Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Moscow). https://doi.org/10.1134/S0006297911110022
Monaselidze JR, Khavinson VKh, Gorgoshidze MZ, et al. (2011). Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-011-1146-x
Khavinson VKh, Lin’kova NS, Tarnovskaya SI. (2016). Short peptides regulate gene expression. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-016-3596-7
Kuzubova NA, Lebedeva ES, Dvorakovskaya IV, et al. (2015). Modulating effect of peptide therapy on the morphofunctional state of bronchial epithelium in rats with obstructive lung pathology. Bulletin of Experimental Biology and Medicine. https://doi.org/10.1007/s10517-015-3047-x
Khavinson VK, Linkova NS, Rudskoy AI, Petukhov MG. (2023). Feasibility of transport of 26 biologically active ultrashort peptides via LAT and PEPT family transporters. Biomolecules. https://doi.org/10.3390/biom13030552
A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity and quality through independent laboratory testing. Every compound from BioLongevity Labs is third-party tested, and its COA is published here, so researchers can review the testing record before a compound enters a protocol.
Each COA reports results from third-party laboratory analysis, including:
Ultra-high-performance liquid chromatography with mass spectrometry (UHPLC-MS) for purity, measured against a 98% or 99% specification depending on the compound
Mass identification for molecular confirmation and content quantitation
Endotoxin testing by Limulus amebocyte lysate (LAL) or recombinant Factor C (rFC) assay, depending on the laboratory
Visual and physical characterization of the finished material
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Scientifically reviewed by Dr. Ky H. Le, MD. Dr. Le is a board-certified family medicine physician with over 20 years of clinical experience. Dr. Le validates the scientific accuracy of all technical content and research citations.
Disclaimer: For Research Purposes Only
This content is provided strictly for research purposes and does not constitute an endorsement or recommendation for the non-laboratory application or improper handling of peptides designed for research. The information, including discussions about specific peptides and their researched benefits, is presented for informational purposes only and must not be construed as health, clinical, or legal guidance, nor an encouragement for non-research use. Peptides described here are solely for use in structured scientific study by authorized individuals. We advise consulting with research experts, medical practitioners, or legal counsel prior to any decisions about obtaining or utilizing these peptides. The expectation of responsible, ethical utilization of this information for legitimate investigative and scholarly objectives is paramount. This notice is dynamic and governs all provided content on research peptides.
Bronchogen (20mg)
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Original price was: $69.97.$69.97Current price is: $69.97.$27.99
